This kit adopts IS6110 and IS1081 dual-target gene detection technology, which significantly improves the detection sensitivity of Mycobacterium tuberculosis. The innovative application of the probe melting curve method realizes the full coverage detection of the rpoB 81bp region of the rifampicin resistance gene, and the determination of drug resistance is more accurate. It is suitable for various sample types such as sputum and alveolar lavage, and can meet the needs of different scenarios such as initial screening in general hospitals, diagnosis and drug resistance analysis in specialized hospitals, and provide reliable technical support for early diagnosis, precise treatment and public health screening of tuberculosis.

Multiplex PCR melting curve technology can be used to simultaneously and rapidly detect the resistance of Mycobacterium tuberculosis to the first-line drug isoniazid (detecting katG and inhA genes) and the important second-line drug fluoroquinolones (detecting gyrA gene), and the whole process from nucleic acid extraction to result interpretation can be completed within 2 hours. The kit has the characteristics of fast detection, high sensitivity and good specificity, which can accurately identify drug-resistant strains in clinical samples, providing an important basis for medication guidance, treatment failure case analysis and treatment plan formulation of MDR-TB patients in drug-resistant tuberculosis patients, and is an ideal detection tool for precise treatment of tuberculosis and drug resistance prevention and control.

Using multiplex PCR melting curve technology, it can accurately identify Mycobacterium, Mycobacterium tuberculosis complex and 7 clinically common non-tuberculous mycobacteria (including Mycobacterium avium, Mycolicibacterium fortuitum, Mycobacteroides abscessus, Mycobacterium kansasii, Mycobacteroides chelonae, Mycobacterium intracellulare and Mycobacterium gordonae) at one time, which can not only accurately distinguish infectious Mycobacterium tuberculosis complex from non-infectious non-tuberculous mycobacteria through specific genetic markers, but also accurately locate to the strain level. The test results can be obtained within 2 hours, providing a fast and accurate etiological diagnosis basis for clinical practice: the prevention and control of infectious diseases and standardized treatment can be initiated in a timely manner for Mycobacterium tuberculosis infection; For non-tuberculous mycobacterial infection, targeted plans can be formulated according to different strains, avoid unnecessary anti-tuberculosis treatment, and are suitable for the detection of various respiratory samples, which is an important tool for the diagnosis, epidemic monitoring and precise treatment of mycobacterial infection