Pure heterozygous identification of transgenic genes - RocGene (Beijing) Technology Co., Ltd.

Application Requirements

As the world's second largest seed demander, the development of the seed industry is of great significance to maintaining China's food security. Following the proposal of the No. 1 Central Committee in 2022 to launch major projects of agricultural biological breeding, the No. 1 document of the Central Committee in 2021, 2022, 2023, 2024 and 2025 emphasizes the promotion of the development of the biological breeding industry for five consecutive years. With the gradual liberalization of the GM policy, the demand for GM testing has increased significantly. As an important step in transgenic breeding, transgenic pure heterozygous detection requires accurate determination of the insertion copy number (single/multiple copies) of foreign genes, which directly affects the evaluation of genetic stability of transgenic crops. Its testing has the characteristics of strong professionalism, high demand for instrument accuracy, and strict requirements for laboratory system.

Solutions

Standard Detection Solution - ArchiPure + RocStation + Archimed R

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., seeds) using 16/32/96 Pro high-throughput fully automated nucleic acid extractors, improving the sensitivity and reliability of subsequent detection.
Assay Preparation: Automated PCR liquid handling system (RocStation) enables plate-based PCR reaction preparation, or Rocgene lyophilized ready-to-use reagents can be applied. Purified nucleic acids after extraction can be transferred in plates simultaneously to improve work efficiency.
Detection Results: 96-well Archimed R series real-time fluorescent quantitative PCR instruments combined with specific primer amplification support absolute quantification, genotyping, melting curve analysis, etc., meeting daily detection needs of standard-throughput experiments.

High-Throughput Detection Solution: ArchiPure 96Pro + RocStation + Archimed R/384

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., seeds) using 96 Pro high-throughput fully automated nucleic acid extractors, improving the sensitivity and reliability of subsequent detection.
Assay Preparation: Automated PCR liquid handling system (RocStation) enables plate-based PCR reaction preparation, or Rocgene lyophilized ready-to-use reagents can be applied. Purified nucleic acids after extraction can be transferred in plates simultaneously to improve work efficiency.
Detection Results: 384-well Archimed R series real-time fluorescent quantitative PCR instruments combined with specific primer amplification support absolute quantification, genotyping, melting curve analysis, etc., meeting daily detection needs of high-throughput experiments.

Application Scenarios

Research Institutes

Used for breeding research to develop new genetically modified traits.

Seed Companies

Develop new genetically modified crop varieties.

Service Companies

Seed testing services.

Detection Workflow

Standard Detection Solution - ArchiPure + RocStation + Archimed R

High-Throughput Detection Solution - ArchiPure 96Pro + RocStation + Archimed R/384

Our Advantages

High-Efficiency Detection

The full product series can be controlled by a single terminal, with fully automated whole-process operation, enabling large-scale sample detection.

Professional Software

Supports function customization with user-friendly Chinese interface. Built-in multiple algorithms with professional absolute/relative quantification and genotyping (SNP) functions, adaptable to the requirements of different detection reagents.

Flexible Solution Design

Customizable complete laboratory design and detection solutions, with one-stop configuration of related instruments and testing services.

Comprehensive After-Sales Service

Lifetime free software upgrades with a professional software team capable of custom development according to user requirements.

Success Cases

Scenario: Relocation and expansion of a testing service company in Beijing.

Requirements: Replace outdated equipment and expand genetically modified (GM) detection capabilities while controlling costs.

Solution:

After purchasing our real-time fluorescent quantitative PCR instrument in the early stage, the laboratory further procured the full range of Archimed products during relocation to a new site, helping to enhance the overall laboratory testing capabilities. The identification range of homozygous/heterozygous results varies with materials of different genetic backgrounds and needs to be corrected based on actual genetic backgrounds. Archimed achieves precise adaptation of result interpretation standards by setting relative quantification parameters.

Results:

Through analysis of early data, more accurate interpretation standards were established by setting relative quantification algorithm parameters. Excellent craftsmanship greatly avoids inter-instrument variation and ensures outstanding detection consistency. The stable performance of the full product series has won recognition from the testing institution.

Laboratory Installation

Experimental Results