Economic animal disease detection - RocGene (Beijing) Technology Co., Ltd.

Application Requirements

Economic animal diseases (such as infectious pancreatic necrosis, foot-and-mouth disease, African swine fever, brucellosis, avian influenza, etc.) are important public health problems that threaten human health and the development of animal farming. Early detection and real-time monitoring of such diseases are of great significance for epidemic prevention and control, public health security and economic loss control of breeding. However, traditional laboratory testing methods are inefficient and complex, and cannot meet the needs of rapid on-site testing and large-scale monitoring.

Solutions

Point-of-Care Testing (POCT) Solution - iFIND

Sample Processing: Built-in ultrasonic combined with chemical lysis + high-efficiency microfiltration membrane purification.
System Preparation: Fully enclosed cartridge with preloaded lyophilized reagents and fully automated system preparation.
Detection Results: Supports multi-sample pooling; thin-film reaction zone with average temperature ramping rate ≥10℃/S; ultra-multiplex detection achieved by multiplex probes + probe melting curve analysis.

Rapid Response Solution - ArchiPure + Archimed Mini/Mini Pro/R

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., nasopharyngeal swabs, oropharyngeal swabs) using fully automated nucleic acid extractor, improving the sensitivity and reliability of subsequent detection.
System Preparation: Manual preparation of detection system or use of Rocgene lyophilized ready-to-use reagents.
Detection Results: Real-time fluorescent quantitative PCR instrument combined with multiplex PCR technology supports amplification and melting curve analysis, standard equipped with 4-color fluorescence and 16/96 throughput, enabling rapid detection of samples with various throughputs.

Mobile Detection Solution - ArchiSuit/Archimed Mini Pro Detection Backpack

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., nasopharyngeal swabs, oropharyngeal swabs) using 16/32 throughput fully automated nucleic acid extractor, improving the sensitivity and reliability of subsequent detection.
System Preparation: Manual preparation of detection system or use of Rocgene lyophilized ready-to-use reagents.
Detection Results: Miniaturized 16-throughput real-time fluorescent quantitative PCR instrument combined with multiplex PCR technology supports amplification and melting curve analysis, standard equipped with 4-color fluorescence, meeting daily detection needs of standard-throughput experiments.

Application Scenarios

Livestock Farms

Monitor livestock infection status (e.g., bovine brucellosis) to prevent the spread of zoonotic diseases. POCT, Rapid Response and Mobile Detection Solutions are recommended.

Wildlife Protection Stations

Detect potential pathogens in wild animals to prevent pathogen transmission to humans. POCT and Mobile Detection Solutions are recommended.

Border Ports and Customs

Rapid screening of pathogens in imported/exported animals and their products. POCT and Rapid Response Solutions are recommended.

Emergency Prevention and Control Sites

Rapidly identify infection sources, assess epidemic scope and guide prevention and control measures during zoonotic disease outbreaks. POCT and Mobile Detection Solutions are recommended.

Detection Workflow

Point-of-Care Testing (POCT) Solution - iFIND

Rapid Response Solution - ArchiPure + Archimed Mini/MiniPro/R

Mobile Detection Solution - ArchiSuit/Archimed Mini Pro Detection Backpack

Our Advantages

High-Efficiency Detection

All solutions support multiplex detection; POCT solution can complete testing within 30 minutes, while Rapid Response and Mobile Detection Solutions save more than 20% of time compared with conventional instruments.

Excellent Sensitivity and Specificity

Combined with automatic nucleic acid extraction and high-efficiency real-time fluorescent quantitative PCR, ensuring high sensitivity and specificity, reducing the risk of false positive/false negative results. It can easily achieve 10/30/50-in-1 pooling detection to lower testing costs.

Wide Applicability

Multiple solutions support various sample types (e.g., swabs, blood, feces, etc.) to meet different requirements.

Flexible Solution Design

Detection reagents can be adapted according to detection throughput requirements with strong adaptability.

Success Cases

Scenario: A large-scale pig breeding enterprise conducts daily environmental monitoring of common diseases to avoid economic losses and control daily breeding risks.

Requirements: The molecular detection laboratory of the breeding enterprise has limited professionalism, and the current platform suffers from severe contamination with high false positive rate of detection results. There is an urgent need to replace it with a molecular platform featuring no manual operation, higher cost-effectiveness, strong anti-contamination capability and accurate detection results.

Solution:

With the POCT solution provided by Rocgene, laboratory personnel only need one-step sample addition with no additional operation required throughout the process. The consumables remain fully enclosed during detection, fundamentally solving the problem of contamination in detection results and replacing the original outdated equipment.

Results:

By collecting environmental samples from the farm, iFIND performed 30-in-1 pooling detection on the results.

On-site Usage Photos

Detection Results