Genetic testing of genetic traits in animals and pets - RocGene (Beijing) Technology Co., Ltd.

Application Requirements

Genetic testing for pet genetic diseases can help identify potential genetic defective genes carried by pets, facilitating early diagnosis, health management, and optimization of breeding plans. With the increasing concern of pet owners about their pets' health and quality of life, the demand for genetic disease testing in the market is growing rapidly. Traditional methods such as serum testing can only identify some diseases, while genetic testing can cover more diseases and provide more accurate results by directly analyzing genetic information.

Solutions

Point-of-Care Testing (POCT) Solution - iFIND

Sample Processing: Built-in ultrasound combined with chemical lysis + high-efficiency microfiltration membrane purification.
System Construction: Fully enclosed cartridge with prefabricated lyophilized reagents and fully automatic system preparation.
Detection Results: Thin-film reaction zone with an average temperature change rate ≥10℃/s. Ultra-multiplex detection realized by multiple probes and probe melting curve analysis.

Open Workstation Solution - Atlas120

Sample Processing: Supports fully automatic nucleic acid extraction for up to 12 samples simultaneously.
System Construction: Open detection reagents, robotic arm for automated reaction system preparation, full-process liquid sealing to avoid contamination.
Detection Results: Standard real-time fluorescent quantitative PCR reaction supporting amplification and melting curve analysis. 6-color fluorescence with 4 amplification detection positions per sample, enabling detection of up to 24 targets.

Rapid Response Solution - ArchiPure+Archimed Mini/Mini Pro/R

Sample Processing: Automatic nucleic acid extractor for high-purity nucleic acid extraction from samples (e.g., nasopharyngeal swabs, throat swabs), improving the sensitivity and reliability of subsequent detection.
System Construction: Manual preparation of detection system or use of Rocgene lyophilized ready-to-use reagents.
Detection Results: Real-time fluorescent quantitative PCR instrument combined with multiplex PCR technology, supporting amplification and melting curve analysis. Standard 4-color fluorescence, 16/96 throughput. Enables rapid detection of samples with various throughputs.

Application Scenarios

Pet Breeding Centers

Before breeding purebred animals (e.g., Persian cats, Golden Retrievers), testing is required for specific genetic disease genes (e.g., feline PKD1 mutation, canine hip dysplasia gene) to prevent the spread of pathogenic genes. Genotyping is performed for susceptible breeds (e.g., Corgis, British Shorthairs) to develop scientific breeding plans and reduce the incidence of genetic diseases in offspring. POCT, Open Workstation, and Rapid Response solutions are recommended based on operational scale.

Pet Hospitals

Health screening for newborn pets: before puppies or kittens are adopted (especially those with unknown origin), test for genetic disease risks (e.g., inherited heart disease in Golden Retrievers, hypertrophic cardiomyopathy in Ragdoll cats). Early warning of latent diseases in young animals to detect high pathogenic risks during the asymptomatic period (e.g., herding dogs with MDR1 gene deficiency) and avoid acute drug-induced poisoning. POCT, Open Workstation, and Rapid Response solutions are recommended based on operational scale.

Third-Party Testing Service Institutions

Provide breed ancestry tracing for mixed-breed pets, determine genetic background, test breed-specific disease risks, and predict susceptibility to diseases, cancer, and drug reactions for small breeding bases or high-value pet owners. POCT, Open Workstation, and Rapid Response solutions can be selected according to clients and sample volume.

Detection Process

Point-of-Care Testing (POCT) Solution - iFIND

Open Workstation Solution - Atlas 120

Rapid Response Solution - ArchiPure+Archimed Mini/MiniPro/R

Our Advantages

High-Efficiency Detection

All solutions support multiplex detection. The POCT solution can complete testing within 1 hour. The Open Workstation and Rapid Response solutions save more than 20% of time compared with conventional instruments.

Excellent Sensitivity & Specificity

Combined with automatic nucleic acid extraction and high-performance real-time fluorescent quantitative PCR, ensuring high sensitivity and strong specificity, reducing false positive/false negative risks, and easily handling trace samples even cell-free DNA from peripheral blood.

Wide Applicability

All solutions support the detection of a variety of genetic diseases and traits to meet diverse needs.

Successful Cases

Scenario: To improve overall pet quality, a pet breeding center needs to avoid high incidence of genetic diseases caused by inbreeding (there are more than 500 canine genetic diseases with an overall incidence >25%). Breeding pets carrying pathogenic genes such as PKD1 (Polycystic Kidney Disease in cats) and CHD (Canine Hip Dysplasia) are excluded to reduce breed-specific disease risks.

Needs: The center is medium-sized with fixed cat and dog breeds, only requiring testing for high-incidence genetic diseases. A cost-effective molecular platform with accurate results is urgently needed.

Solution:

With the Rapid Response Solution provided by Rocgene, trained breeders can complete the entire test within 1 hour. Using pet blood, oral swabs, or even saliva as samples, nucleic acid extraction is completed within 15 minutes with ArchiPure 16, and genotyping is finished within 45 minutes with Archimed 16.

Result Display:

The pet breeding center successfully distinguished the genotypes of breeding animals using Archimed 16: the female was CC genotype and the male was CA genotype. Their offspring will not develop polycystic kidney disease but have a 50% chance of being carriers of the pathogenic gene.

On-site Application Photo

Test Result