Microbial community analysis - RocGene (Beijing) Technology Co., Ltd.

Application Requirements

Microbiome and environmental DNA (eDNA) analysis are important areas in biology and ecology research in recent years, aiming to reveal the diversity of microbial communities and their role in ecosystems by analyzing environmental DNA samples. Traditional culture methods can only isolate some microorganisms, and the 16s and mNGS sequencing results require high specificity, accurate quantification of low-abundance strains, and effective technology to reduce PCR amplification bias.

Solutions

Automated Detection Solution - ArchiPure Series Extraction + Archimed R Series + qPCR Premix

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., feces, environmental swabs) using 16/32/96-throughput fully automated nucleic acid extractor, improving the sensitivity and reliability of subsequent detection.
Supporting Reagents: High-sensitivity PCR reaction Mix, improving detection result accuracy and effectively reducing PCR amplification bias.
Detection Platform: 1.33× higher sensitivity; adjustable temperature ramping rate ensures more accurate detection results for different samples; multiplex probe-based relative/absolute quantification achieves ultra-high detection efficiency.

Mobile Detection Solution - ArchiSuit + qPCR Premix

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., feces, environmental swabs) using 16-throughput fully automated nucleic acid extractor, improving the sensitivity and reliability of subsequent detection.
Supporting Reagents: High-sensitivity PCR reaction Mix, improving detection result accuracy and effectively reducing PCR amplification bias.
Detection Platform: Mini16 series real-time fluorescent quantitative PCR instrument enables ultra-rapid and high-precision qualitative/quantitative detection of microorganisms; combined with mobile power supply and auxiliary equipment in an aviation case, it can complete rapid nucleic acid detection on-site.

Detection Performance Enhancement Solution - ArchiPure Series + ArchiCycler Series + Archimed R Series + qPCR Premix

Primer Validation: ArchiCycler gradient PCR supports user-defined annealing temperature with physical isolation and ±0.1℃ high-precision temperature control, enabling efficient confirmation of the optimal annealing temperature for primers.
Supporting Reagents: High-sensitivity PCR reaction Mix, improving detection result accuracy.
Detection Platform: 1.33× higher sensitivity; adjustable temperature ramping rate ensures more accurate detection results for different samples; multiplex probe-based relative/absolute quantification achieves ultra-high detection efficiency.

Application Scenarios

Environmental Monitoring and Protection

Analyze microbial communities in different environments (e.g., water bodies, soil, air), assess ecological health status, and monitor pollutants and changes in microbial diversity.

Biodiversity Survey and Species Identification

Non-invasive detection of microorganisms and animals in water, soil, air and other environments through environmental DNA (eDNA) analysis for species identification and monitoring of endangered species.

Agriculture and Food Safety

In agriculture, microbiomics is used for soil health assessment, pathogen monitoring and crop health management. In the food industry, environmental DNA (eDNA) is used to detect food contamination sources and monitor food safety.

Ecological Restoration and Pollution Control

Use environmental DNA (eDNA) technology to monitor changes in microbial communities during pollution control, providing data support for evaluating the effectiveness of pollution control measures.

Disease Surveillance and Pathogen Detection

Monitor pathogenic microorganisms in public health environments (especially in water sources, food and air) through environmental DNA (eDNA) analysis to quickly identify potential health threats.

Detection Workflow

Automated Detection Solution - ArchiPure Series Extraction + Archimed R Series + qPCR Premix

Mobile Detection Solution - ArchiSuit + qPCR Premix

Performance Enhancement Solution - ArchiCycler Series + Archimed R Series + qPCR Premix

Our Advantages

High-Efficiency Nucleic Acid Extraction

ArchiPure optimizes the extraction process to obtain more intact nucleic acid from environmental samples, reduce interference from potential contaminants, efficiently extract trace nucleic acid from environmental samples, ensure sample quality and purity, adapt to extraction requirements in complex environmental samples, and improve analysis accuracy.

Flexible Detection Solutions

Archimed series real-time fluorescent quantitative PCR instruments are available in multiple specifications including 4/5/6 channels and 16/96/384 throughput, which can simultaneously detect multiple microorganisms or pathogens qualitatively or quantitatively, provide richer information, and meet high-throughput analysis needs.

Excellent Sensitivity and Specificity

High-performance self-developed enzyme system, combined with ultra-fast temperature ramping rate and 1.33× resolution instrument sensitivity, ensures high sensitivity and specificity of results.

Wide Applicability

Gradient temperature can be edited independently; physical isolation design ensures the performance of optimizing primer reaction conditions; ±0.1℃ temperature accuracy + Tube temperature control mode is more suitable for the needs of micro-volume reactions.

Success Cases

Scenario: A research project in Beijing on the gut microbial community of children with Autism Spectrum Disorder (ASD) aimed to screen differentially expressed bacterial genera through sequencing technology and evaluate the efficacy of probiotic therapy by observing changes in differential expression.

Requirements: For fecal samples, researchers needed a method for dynamically detecting changes in the absolute expression levels of specific genes to evaluate the intervention effect of probiotics, and explore the clinical efficacy of new therapies combined with children's behavioral scores.

Solution:

Researchers screened differentially expressed genes from 16S sequencing results, extracted nucleic acid from children's fecal samples using ArchiPure32, used high-sensitivity qPCR premix provided by Rocgene, and verified the probiotic intervention results in children with Archimed R6's relative quantification (double standard curve method).

Results:

Relative quantification (double standard curve method) results can not only accurately evaluate the content of different bacterial genera in each sample, but also reflect changes in the relative expression values of different bacterial genera. Combined with children's behavioral scores, it accurately reflects the impact of probiotics on children with autism. It also demonstrates the important value of Rocgene series instruments in the validation of gene expression quantification in microbiology-related research.

On-site Usage Photos

Detection Results