Syndrome Rapid differential detection of multiple pathogens - RocGene (Beijing) Technology Co., Ltd.

Application Requirements

The CDC and sentinel hospitals undertake the task of infectious disease monitoring, prevention and control. The CDC focuses on monitoring the epidemic situation, and sentinel hospitals can quickly detect cases by virtue of their diagnosis and treatment advantages. Both are in urgent need of rapid identification technology to achieve early diagnosis and treatment and curb the spread of diseases. Existing technologies such as traditional culture methods take a long time, immunology and conventional qPCR detection techniques have limited targets and low diagnostic efficiency. Although new technologies such as multiplex PCR and microfluidics have made progress, they still cannot fully meet the actual needs in terms of detection throughput, cost, and grassroots adaptability. Application units urgently need a molecular detection platform with high detection throughput, fast speed, support for ultra-many detections, and high cost performance.

Solutions

Point-of-Care Testing (POCT) Solution - iFIND

Sample Processing: Built-in ultrasonic combined with chemical lysis + high-efficiency microfiltration membrane purification.
System Preparation: Fully enclosed cartridge with preloaded lyophilized reagents and fully automated system preparation.
Detection Results: Thin-film reaction zone with average temperature ramping rate ≥10℃/S; ultra-multiplex detection achieved by multiplex probes + probe melting curve + multi-cartridge combination.

Open Workstation Solution - Atlas120

Sample Processing: Supports fully automated nucleic acid extraction for up to 12 samples simultaneously.
System Preparation: Open detection reagents with robotic arm for automated reaction system preparation, full-process liquid sealing to avoid contamination.
Detection Results: Standard real-time fluorescent quantitative PCR reaction supporting amplification and melting curve analysis; 6-color fluorescence with 4 amplification detection positions per sample, enabling detection of 24 targets.

Standard Detection Solution - ArchiPure + Archimed X Series

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., nasopharyngeal swabs, oropharyngeal swabs) using 16/32/96 Pro throughput fully automated nucleic acid extractor, improving the sensitivity and reliability of subsequent detection.
System Preparation: Manual preparation of detection system or use of Rocgene lyophilized ready-to-use reagents.
Detection Results: 96-well Archimed X series real-time fluorescent quantitative PCR instrument combined with multiplex PCR technology supports amplification and melting curve analysis, meeting daily detection needs of standard-throughput experiments.

Application Scenarios

Provincial/Municipal Centers for Disease Control and Prevention (CDC)

Undertake regional pathogen monitoring and verification, and establish multi-syndrome detection systems;

County-level Centers for Disease Control and Prevention (CDC)

Responsible for rapid grassroots detection and carry out monitoring tasks of the National Pathogen Identification Network;

Sentinel Hospitals

As the core of front-end detection, sample source guarantee and data support in the National Pathogen Identification Network monitoring, responsible for early case detection and reporting, standardized sample collection and submission, clinical data linkage supplementation and grassroots prevention and control response.

Detection Workflow

Point-of-Care Testing (POCT) Solution - iFIND

Open Workstation Solution - Atlas 120

Standard Detection Solution - ArchiPure + Archimed X Series

Our Advantages

High-Efficiency Detection

All solutions support ultra-multiplex detection; POCT solution can complete virus-related detection within 30 minutes, while Workstation and Standard Detection Solutions save more than 20% of time compared with conventional instruments.

Excellent Sensitivity and Specificity

Combined with automatic nucleic acid extraction and high-efficiency real-time fluorescent quantitative PCR, ensuring high sensitivity and specificity, reducing the risk of false positive/false negative results.

Wide Applicability

Multiple solutions support various sample types (e.g., nasopharyngeal swabs, oropharyngeal swabs, feces, etc.) to meet different requirements.

Flexible Solution Design

Detectable pathogen types can be customized according to market demands with strong adaptability. POCT and Open Workstation Solutions support detection target splitting, easily meeting personalized needs such as low-plex detection.

Success Cases

Requirements: A municipal CDC in Southwest China focuses on epidemic monitoring, precise prevention and control, and technical support: coordinating regional testing resources and formulating standardized detection protocols; receiving samples submitted by sentinel hospitals for verification and in-depth traceability (e.g., genotyping, drug resistance analysis); rapidly confirming pathogen types and characteristics to achieve early epidemic warning; curbing cross-border/cross-regional transmission of pathogens and reducing public health risks. The user required a molecular detection platform covering multiple pathogen types, flexible target combinations, simple experimental operation and fast response speed.

Solution:

Through pre-sales recommendation, the user learned about our independently developed molecular POCT platform iFIND. After technical communication and rigorous performance testing, ultra-multiplex etiological detection was performed on patients with diarrhea symptoms using fecal/anal swab samples combined with multiplex detection reagent cartridges.

Results:

Rapid detection of diarrhea syndrome was completed within 90 minutes using the POCT solution.

On-site Usage Photos

Detection Results